FastQCFastQC Report
Tue 27 Sep 2016
WNN_14124_13988_CGTACT_read2_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_14124_13988_CGTACT_read2_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67330824
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2178210.3235085909538252No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1973130.29305003010211195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA154950.021.81068444
GTCCTAC334950.019.9099921
ACTGCGA81650.019.8707858
CAACGCG51350.019.60817134
TCTTACG65750.019.54279744
ATTTCGT180200.019.18933342
ACGAACG48200.019.15687629
GTAGGAC305750.018.9812323
TTTCGTC179950.018.93805343
CGAACGG48650.018.93448830
GATTTCG182650.018.85810741
TAGGGCG66050.018.8057931
TAGACCG40550.018.5074464
TACGAAC51100.018.41647728
TTAGTCG48850.018.36674739
TAGGACC178600.018.1265094
TCGTCCC81600.018.02682737
TAGACGC61550.017.5206814
GCGTTTA72200.017.32695635
CGAGAAA196400.017.31930721
Produced by FastQC (version 0.11.2)