FastQCFastQC Report
Tue 27 Sep 2016
WNN_14124_13988_CGTACT_read1_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_14124_13988_CGTACT_read1_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67330824
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2350320.3490704346645156No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1945460.2889404710092364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA145000.021.21828544
CGAACGG47850.021.03676830
ACTGCGA83100.020.4688328
TTAGTCG46000.020.2109639
GTAGGAC329300.020.1483
TCTTACG64300.019.51530844
ACGAACG51600.019.33718529
GTCCTAC326200.019.2756861
CAACGCG53000.019.20717234
TAGACGC67600.018.8556654
GATTTCG172400.018.7282741
TAGGACC193800.018.7220734
TACGAAC54100.018.61059828
ATTTCGT172950.018.49079542
TTTCGTC171300.018.33543
CGAGAAA216800.018.32547621
ATCACGG196850.018.1988435
AATCACG199550.018.0977534
TGCGACC57500.018.03878610
GCGAGAA221850.017.80901320
Produced by FastQC (version 0.11.2)