FastQCFastQC Report
Tue 27 Sep 2016
WNN_14121_13987_TAAGGC_read2_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_14121_13987_TAAGGC_read2_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71807985
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2616190.36433134838695164No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG2320630.3231715804307836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA221550.023.6062144
GTCCTAC434550.021.7661551
GATTTCG259400.021.05355541
TTTCGTC254600.020.81665443
ATTTCGT259350.020.79675942
TAGGACC212100.019.954344
GTAGGAC414050.019.8693543
CTAGACG44450.019.3598613
ACGAACG49400.019.35859329
TAGACGC71550.019.1943634
CGAACGG50800.018.73853530
ACTGCGA80650.018.4256028
ATCACGG243950.018.31308735
TCACGTT278400.018.3071527
TAGGACG255900.018.1988584
TCTTACG66700.018.17584644
CGAGAAA269900.018.17085821
AATCACG249450.018.12810134
CACGTTT292800.018.0672528
TGGCGAG390800.017.99579818
Produced by FastQC (version 0.11.2)