FastQCFastQC Report
Tue 27 Sep 2016
WNN_14121_13987_TAAGGC_read1_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_14121_13987_TAAGGC_read1_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71807985
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2833960.3946580592673642No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG2273960.3166723032264448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA201450.022.99692744
TAGGACC219600.021.12614
GTCCTAC415650.020.7474481
GATTTCG234750.020.59917341
GTAGGAC436850.020.5605983
TTTCGTC233800.020.04316743
ATTTCGT241800.019.94403542
ACGAACG49000.019.41858129
TAGACCG42150.019.3958514
TAGACGC73100.019.391254
ATCACGG254850.018.98153335
CGAACGG50450.018.81680730
ACTGCGA84250.018.8072058
CTAGACG44400.018.6604373
AATCACG263650.018.61992534
TCTTACG62350.018.5050144
CGAGAAA291500.018.47174321
TACGAAC52300.018.32458328
TGGCGAG413900.017.98802818
ACGGAAA273350.017.88292138
Produced by FastQC (version 0.11.2)