FastQCFastQC Report
Tue 27 Sep 2016
WEN_A_P4_12836_AGGCAGAA_read2_50k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_A_P4_12836_AGGCAGAA_read2_50k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74531185
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC984040.13203063925523256No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG862270.11569251179892014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA120400.018.5787344
ATTTCGT124750.018.40714542
GTCCTAC245800.017.8788431
GATTTCG127400.017.76454541
TTTCGTC129300.017.45316343
GTAGGAC230000.016.7843763
TATGGCG178000.015.62481516
TAGCCCT343750.015.4256374
TAGGACG121150.014.7709874
GGTCTAC236650.014.6219981
AATCACG141250.014.24466434
GTGTAGC288050.014.2337551
TCACGTT158450.014.18365227
TGTACTG348600.014.0255255
ACGTTTT166300.013.97779829
CGGAAAA147750.013.90178539
GTATAGC218900.013.6885871
ATCACGG144750.013.62653535
TATACTG194250.013.5696885
GTAGCCC256650.013.559643
Produced by FastQC (version 0.11.2)