FastQCFastQC Report
Tue 27 Sep 2016
WEN_A_P4_12836_AGGCAGAA_read1_50k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_A_P4_12836_AGGCAGAA_read1_50k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74531185
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1033680.13869093856484369No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG885840.1188549464227625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA118050.018.56497844
GATTTCG123500.018.26266341
ATTTCGT122950.018.23690242
GTAGGAC238300.017.7948993
GTCCTAC244200.017.1768261
TTTCGTC128100.017.09158343
TAGGACG125050.016.2342534
TATGGCG187550.016.08969516
TAGCCCT348800.016.0292554
GTGTAGC299050.014.6074121
GTAGCCC263150.014.484643
TGTACTG350500.014.3450625
AATCACG144100.014.263494534
CGAGAAA158650.014.17167821
CGGAAAA149400.014.0508839
GTATAGC216350.013.9690761
TACACTG342900.013.9317795
ATCACGG146850.013.8016835
ACGGAAA150500.013.7878938
TATACTG198450.013.7541885
Produced by FastQC (version 0.11.2)