FastQCFastQC Report
Tue 27 Sep 2016
WEN_13114_13990_TCCTGA_read2_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_13114_13990_TCCTGA_read2_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69912828
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC798190.11416931954175849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA336850.019.22997944
GTCCTAC522250.018.461031
GATTTCG358100.018.37737341
TTTCGTC366100.017.80040643
ATTTCGT371750.017.7988142
GTAGGAC504250.017.6720033
TAGGACG478500.017.243234
TAGGACC209050.016.4022124
AGGACGT500450.016.19365
GGACGTG486900.016.1703956
TGTAGGA594450.015.9131012
CTGTAGG599350.015.2932831
TATGGCG616800.015.292047516
GACGTGA270550.015.2388617
TCCTACA626200.015.057072
TTAGGAC351800.014.82016853
ATGGCGA654150.014.76521417
TGGCGAG634650.014.75413118
AGTGTGC559600.014.6305988
CCTACAG620000.014.6070343
Produced by FastQC (version 0.11.2)