FastQCFastQC Report
Tue 27 Sep 2016
WEN_13114_13990_TCCTGA_read1_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_13114_13990_TCCTGA_read1_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69912828
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC832420.1190654167215207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA312950.018.61539644
GTAGGAC535800.018.2886493
GATTTCG338050.017.55477341
GTCCTAC522700.017.5530591
TTTCGTC340300.017.302743
ATTTCGT349900.017.0858842
TAGGACG503200.016.9142884
TGTAGGA609000.016.7868522
TAGGACC224150.016.6669854
CTGTAGG622100.015.9361971
GGACGTG504500.015.9078276
AGGACGT523250.015.8921695
TCGTCAA115700.015.5559812
TATGGCG634450.015.36306516
GTCGTCA125700.014.9236391
TGGCGAG654800.014.81834418
GACGTGA273050.014.7603367
ATGGCGA676600.014.72822717
ATCACGG383900.014.70598135
CGGAAAA400800.014.69638339
Produced by FastQC (version 0.11.2)