FastQCFastQC Report
Tue 27 Sep 2016
WEN_13111_13989_AGGCAG_read2_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_13111_13989_AGGCAG_read2_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84290461
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC959590.11384324971244374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA344600.020.13151444
GATTTCG366200.019.32816141
TTTCGTC373200.018.84075743
ATTTCGT383250.018.68818542
GTCCTAC566450.018.2635821
GTAGGAC529750.017.6775443
TAGGACC247400.017.1159424
TAGGACG470550.016.215994
TCGTCAA123450.015.5786522
TATGGCG607600.015.559875516
TGTAGGA629200.015.3702072
GGACGTG476100.015.1877986
AGGACGT492700.015.1578025
GTCGTCA137150.014.9675511
TGGCGAG634400.014.91966218
ATCACGG372100.014.88348935
ATGGCGA650300.014.8798217
AGTGTGC618150.014.7942768
CGGAAAA388250.014.7541639
TCCTACA686850.014.7112472
Produced by FastQC (version 0.11.2)