FastQCFastQC Report
Tue 27 Sep 2016
WEN_13111_13989_AGGCAG_read1_100k_pyramidal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN_13111_13989_AGGCAG_read1_100k_pyramidal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84290461
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1008140.11960309482706472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA314600.018.81149744
GTAGGAC578750.018.1163563
GATTTCG333050.017.91730141
GTCCTAC539100.017.8525661
TTTCGTC341700.017.45050643
ATTTCGT349000.017.3377642
TAGGACC262450.016.7637024
TAGGACG504950.016.3162174
TGTAGGA672850.016.1575722
TCGTCAA121500.015.4647272
GGACGTG507950.015.3587646
AGGACGT525350.015.3520425
CTGTAGG694350.015.2831021
CGGAAAA399200.015.17377739
ATCACGG389100.015.1151535
TATGGCG644100.015.03370416
GTCGTCA130950.015.0317731
ACGGAAA407550.014.81435538
AATCACG406400.014.72617234
TCACGGA406750.014.53553536
Produced by FastQC (version 0.11.2)