FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_C_14724_AGGCAGAA_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_C_14724_AGGCAGAA_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87259819
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1042390.11945818957062011No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG920520.10549185301427225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA197400.026.26389544
GATTTCG203600.026.04492441
ATTTCGT215950.024.66594742
TTTCGTC217750.024.40099343
GTAGGAC387650.023.1656283
GTCCTAC430500.023.1321431
TAGGACG197750.022.2778384
TATGGCG298400.022.15899816
CGGAAAA223750.020.47609339
ATGGCGA342800.020.1577317
GCGAGAA248800.020.04247520
AATCACG224550.019.8316234
AGGACGT218750.019.7874345
TGGCGAG341450.019.59489418
ATCACGG223200.019.56674835
CGAGAAA259450.019.55951321
TCACGTT266000.019.34181827
ACGGAAA232100.019.22373238
CACGTTT281100.019.16599528
ACGTTTT281600.019.1185729
Produced by FastQC (version 0.11.2)