FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_C_14724_AGGCAGAA_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_C_14724_AGGCAGAA_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87259819
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1174630.1346129310673908No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG949890.10885766334216211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA177900.025.81743244
GATTTCG189300.025.44984241
ATTTCGT196500.024.3605142
GTAGGAC430700.023.9664823
TTTCGTC195850.023.93544243
TATGGCG321300.022.97589516
GTCCTAC414800.022.461941
TAGGACG217350.022.2673764
CGGAAAA241500.021.77150539
AATCACG244100.021.10022734
ATGGCGA365300.021.0334317
GCGAGAA270600.021.02954320
CGAGAAA281300.020.7300921
ACGGAAA249600.020.67678338
ATCACGG243750.020.60885835
TGGCGAG368550.020.22722618
TCACGGA252400.019.99230236
TAGGACC287350.019.9205864
GGCGAGA284300.019.83820319
AGGACGT239000.019.6407345
Produced by FastQC (version 0.11.2)