FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_B_14726_CGTACTAG_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_B_14726_CGTACTAG_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105557821
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2030390.1923486086360195No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1887480.1788100570965746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA210150.024.6356144
ATTTCGT218450.024.06656642
GATTTCG218750.023.9943641
TTTCGTC220600.023.77187243
GTAGGAC401200.023.7498823
GTCCTAC448250.023.3761371
TAGGACG167200.021.9934314
TATGGCG287050.021.84333216
AATCACG224950.019.57857334
TAGGACC287700.019.2146034
CGGAAAA235200.018.98040639
TCACGTT259650.018.94140827
ACGTTTT273500.018.91187729
CGAGAAA259100.018.9034521
ACGGAAA233900.018.82765238
CACGTTT276050.018.6479728
ATCACGG235250.018.3843735
GCGAGAA261150.018.32166320
ATGGCGA360500.018.2898817
TTTCACG299600.018.13919325
Produced by FastQC (version 0.11.2)