FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_B_14726_CGTACTAG_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_B_14726_CGTACTAG_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105557821
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC2200960.2085075249895505No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1874290.1775605049672255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC426250.024.3354193
TTCGTCA185100.024.04764244
GATTTCG193900.023.78659641
ATTTCGT193800.023.5245742
TTTCGTC196250.022.89440243
GTCCTAC430550.022.683241
TATGGCG296000.022.13181516
TAGGACG183350.021.6202474
AATCACG232650.020.37106734
TAGGACC299250.020.1861344
CGAGAAA267600.020.01690721
CGGAAAA245200.019.87769939
ACGGAAA247200.019.5658238
GCGAGAA270000.019.27680620
ATCACGG243250.019.1507635
ATGGCGA366000.018.8906717
TGTAGGA553950.018.7775232
TCACGGA248650.018.69948636
TGGCGAG361950.018.53682318
ATATGGC591550.018.32967415
Produced by FastQC (version 0.11.2)