FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_A_14722_TCCTGAGC_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_A_14722_TCCTGAGC_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79595950
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT113150.018.9670442
GATTTCG114600.018.68963841
GTCCTAC254800.018.6525961
TTCGTCA112000.018.54807544
GTAGGAC221550.018.4058533
TTTCGTC116600.018.15979643
TATGGCG146500.016.81143216
GGTCTAC274350.016.5542071
TAGGACG92500.016.2791844
TAGCCCT387900.016.2306924
GTGTAGC318700.014.8092471
TCGAAAA111550.014.73070724
AATCACG120300.014.718834
TCACGTT140500.014.59110327
TACACTG358350.014.5448075
GTAGCCC298500.014.5153023
TAGGACC171150.014.4240324
GTATAGC219950.014.1721331
TATACTG202150.014.0716295
ACGTTTT153000.014.02717729
Produced by FastQC (version 0.11.2)