FastQCFastQC Report
Tue 27 Sep 2016
P4_WNN_A_14722_TCCTGAGC_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WNN_A_14722_TCCTGAGC_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79595950
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC245450.019.2165433
TTCGTCA100200.017.72463844
GATTTCG105950.017.42849341
GTCCTAC246850.017.3701781
TATGGCG156950.017.35076316
ATTTCGT105650.017.22842
TAGCCCT403150.016.844834
TTTCGTC107350.016.77051243
AATCACG127150.016.66343134
TAGGACG100450.016.4469994
GGTCTAC253050.015.9100051
ATCACGG133350.015.42808735
TAGGACC181050.015.2004144
GTAGCCC317400.015.1861963
CGAGAAA149900.015.0558421
GTGTAGC336250.014.8848581
TATACTG200650.014.66817955
GTATAGC222300.014.6667641
TACACTG364300.014.624275
CGGAAAA144500.014.60659739
Produced by FastQC (version 0.11.2)