FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_C_5_14727_TCCTGAGC_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_C_5_14727_TCCTGAGC_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93629010
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC3448390.3683035845407316No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG3237520.34578171872158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA209050.027.85040344
GATTTCG220200.027.22565341
ATTTCGT226600.026.5429842
TTTCGTC229100.025.90703243
TATGGCG304550.024.52926616
GTAGGAC409900.024.5217653
GTCCTAC457850.024.1582011
TAGGACG200600.022.8675654
CGGAAAA232250.021.88633339
AATCACG226850.021.88204234
ATGGCGA358900.021.7647817
GCGAGAA257950.021.74551220
CGAGAAA264450.021.52345521
TGGCGAG355950.021.2415718
ACGTTTT282600.021.15336629
ATCACGG229750.021.10730235
TCACGTT271950.020.91560427
ACGGAAA238400.020.91177738
CACGTTT291100.020.4883928
CGTTTTT286950.020.45316330
Produced by FastQC (version 0.11.2)