FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_C_5_14727_TCCTGAGC_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_C_5_14727_TCCTGAGC_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93629010
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC3868360.4131582722064454No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG3116880.3328968233243094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG200700.027.31154341
TTCGTCA195200.027.21071844
ATTTCGT208350.026.45455442
TTTCGTC208200.025.92389943
GTAGGAC440900.025.1884923
TATGGCG323200.024.95190216
TAGGACG208700.024.106454
GTCCTAC430900.023.497321
GCGAGAA270300.022.6897320
CGGAAAA246700.022.65583439
AATCACG241050.022.49087734
ATGGCGA375700.022.45467417
CGAGAAA277650.022.40599821
ATCACGG244400.021.8698635
ACGGAAA250100.021.83783538
TGGCGAG372050.021.82945818
TCACGGA254500.021.04526136
TAGGACC311350.020.341474
AGGACGT239550.020.276155
GGCGAGA296950.020.24598319
Produced by FastQC (version 0.11.2)