FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_B_4_14723_TAAGGCGA_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_B_4_14723_TAAGGCGA_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133238260
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG207450.020.73377641
TTCGTCA200900.020.65870544
ATTTCGT215350.019.92096742
GTCCTAC454200.019.5290831
TTTCGTC217150.019.44112243
TATGGCG293500.019.0830416
GTAGGAC408050.018.4784893
AATCACG224300.017.05528634
TAGGACG191500.016.8172344
ATGGCGA367450.015.88497917
ATCACGG236600.015.81492435
TAGCCCT651750.015.6199444
TCACGTT258100.015.59592427
CGAGAAA260950.015.56952221
ACGTTTT280400.015.49561129
GGTCTAC458300.015.4595151
CGGAAAA251800.015.36252139
CACGTTT282900.015.17013128
TGGCGAG374400.015.12127718
ACGGAAA253900.015.09242438
Produced by FastQC (version 0.11.2)