FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_B_4_14723_TAAGGCGA_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_B_4_14723_TAAGGCGA_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133238260
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA184300.020.88511844
GATTTCG192100.020.87458841
TATGGCG307850.020.62851716
ATTTCGT199500.019.98997542
GTAGGAC434100.019.9672833
TTTCGTC197050.019.84734343
TAGGACG203650.019.1846754
AATCACG243100.018.40855834
GTCCTAC449400.018.1130581
ATCACGG253150.017.41010935
ATGGCGA383300.017.2565917
CGAGAAA284600.016.9729721
CGGAAAA271000.016.86814739
TGGCGAG389050.016.56618718
ACGGAAA272800.016.46620438
GCGAGAA291400.016.33533720
TAGCCCT678150.016.3201624
TCACGGA276450.015.80948336
TCACGTT247250.015.6313527
ATATGGC642850.015.47666315
Produced by FastQC (version 0.11.2)