FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_A_14725_AGGCAGAA_read2_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_A_14725_AGGCAGAA_read2_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93063022
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC989270.10630108272220089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA163550.024.56116344
GATTTCG172750.023.79395541
ATTTCGT179300.023.0588442
TTTCGTC176900.022.97317943
GTCCTAC368100.021.3959471
GTAGGAC336600.021.3534643
TATGGCG241900.020.91251216
TAGGACG158900.019.5042784
AATCACG184650.018.24369434
ATGGCGA290150.018.22343817
CGAGAAA210700.018.0687621
TGGCGAG289900.017.61763818
GGTCTAC339000.017.4357911
ACGGAAA198500.017.33545938
CGGAAAA200050.017.32802839
GCGAGAA219050.017.32578720
ATCACGG189950.017.3056435
TCACGTT219950.017.20016327
TAGGACC242050.017.0630384
TAGCCCT486150.017.0362994
Produced by FastQC (version 0.11.2)