FastQCFastQC Report
Tue 27 Sep 2016
P4_WEN_A_14725_AGGCAGAA_read1_200k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4_WEN_A_14725_AGGCAGAA_read1_200k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93063022
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1089340.1170540109905307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG161400.023.57278841
TTCGTCA156300.023.42512144
GTAGGAC355050.022.542033
TTTCGTC164450.022.49165343
ATTTCGT170550.022.1775542
TATGGCG258750.021.38166416
GTCCTAC360400.021.0119781
TAGGACG167400.020.5397034
AATCACG202500.019.38391534
ATCACGG203550.019.0372935
ATGGCGA305300.018.89972117
CGAGAAA235650.018.58607721
CGGAAAA218750.018.5089839
TGGCGAG304050.018.47820518
ACGGAAA216250.018.37721638
GCGAGAA231200.018.26826720
TAGGACC253050.018.0646634
TCACGTT203150.017.70455727
TAGCCCT494350.017.5550924
TCACGGA221200.017.4884736
Produced by FastQC (version 0.11.2)