FastQCFastQC Report
Tue 1 Mar 2016
k36me3_WNN3_7519_ACTGAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamek36me3_WNN3_7519_ACTGAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50686201
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT25150.029.8443091
CTCGTAT24450.025.82247744
CGGTATT34750.025.13351427
TTCGGTA35350.025.07953325
TCGGTAT35300.024.74113526
TAAGCCG48650.024.46104216
AGCCGAT49400.024.35628718
CCGATGA50900.023.68173420
ATTCGGT38200.023.20841824
AATTCGG37850.023.1905423
TCTCGTA28250.022.89677243
CGATGAC55750.021.7030321
GAATTCG40850.021.54129222
TCATAAC61900.019.6553129
TATCTCG33900.019.40765441
ATTCGTT40950.019.0265562
ACGTCAT65650.018.6313326
GCGGTCT49450.017.97697439
AAGCCGA67300.017.97621717
ATGACGT67850.017.96237423
Produced by FastQC (version 0.11.2)