FastQCFastQC Report
Tue 1 Mar 2016
k36me3_WEN3_7515_GTGAAA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamek36me3_WEN3_7515_GTGAAA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48591931
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT38500.033.8881841
CGTCATA66850.028.33210427
CGGTATT54400.027.9014827
TCGGTAT55100.027.62566626
TAAGCCG69900.027.18672416
TTCGGTA56650.026.7922825
AATTCGG58250.026.32069623
CTCGTAT29850.026.30803544
CCGATGA73950.025.90564720
AGCCGAT73800.025.868618
AACGATC29900.025.82730738
ATTCGGT60250.025.19141424
CGATGAC76850.025.0165121
GAATTCG61350.024.88315422
ACGTCAT77200.024.78907226
ATTCGTT54700.024.2943022
ATGACGT78850.024.15887623
TTCGTTG55800.023.5360093
TCTCGTA33500.023.50962343
TCATAAC84550.022.68794429
Produced by FastQC (version 0.11.2)