FastQCFastQC Report
Tue 1 Mar 2016
input_WNN3_7377_ACTTGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WNN3_7377_ACTTGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42449195
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35650.029.6115642
TCGTATG36750.028.8449243
TCTCGTA37800.027.86912241
CGTATGC42600.024.99320844
AATCTCG48400.021.99275239
ATCTCGT51900.020.72149740
CGTCTGA69100.015.81800816
ATCGGAA70750.015.3261432
GATCGGA70950.015.197431
ACACGTC75250.014.61302213
ACGTCTG75650.014.477509515
TTGAATC87300.012.69726636
CACGACG11900.011.64305815
TCGGAAG95750.011.6007473
CACGTCT101200.011.17007414
CGGAAGA102400.010.7612374
TGAATCT110400.010.2591237
GAATCTC110850.010.25733338
CACACGT113500.010.09528112
CTTGAAT114300.09.89242735
Produced by FastQC (version 0.11.2)