FastQCFastQC Report
Tue 1 Mar 2016
input_WNN2_6153_CTTGTA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WNN2_6153_CTTGTA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54700924
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4921610.8997306882786844TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT540250.042.33891342
TCGTATG541750.042.24194743
TCTCGTA542450.042.15893641
CGTATGC543100.042.11696244
AATCTCG554650.041.42993539
ATCTCGT556650.041.1190440
GTAATCT581850.039.40464837
TTGTAAT595150.038.6050635
TGTAATC603550.038.06780636
TAATCTC613100.037.5053238
CCTTGTA627150.036.7580333
CTTGTAA628050.036.6738334
GATCGGA646050.036.0823331
ATCGGAA651400.035.9468572
CGTCTGA651350.035.94289416
ACGTCTG664300.035.27202215
ACACGTC666700.035.2308413
ACCTTGT664500.034.68532632
TCGGAAG676750.034.6167563
CGGAAGA685050.034.332224
Produced by FastQC (version 0.11.2)