FastQCFastQC Report
Tue 1 Mar 2016
input_WNN1_6146_ATCACG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WNN1_6146_ATCACG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33130392
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC3614781.0910767370334766TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT398250.042.49840542
TCTCGTA399100.042.430241
TCGTATG399300.042.40894343
CGTATGC401550.042.1939344
ACGATCT406400.041.74917237
CACGATC408750.041.52472736
CGATCTC409150.041.44718638
ATCTCGT409750.041.33817740
TCACGAT411450.041.26827635
GATCTCG414800.040.97278639
CATCACG419350.040.4800433
ATCACGA419350.040.44356534
CGTCTGA439100.039.15526616
ATCGGAA446050.038.9200942
GATCGGA446400.038.8994331
ACGTCTG443300.038.7793315
ACACGTC448200.038.4535313
TCGGAAG460450.037.7507483
CGGAAGA465100.037.378054
GTCACAT456300.037.11047429
Produced by FastQC (version 0.11.2)