FastQCFastQC Report
Tue 1 Mar 2016
input_WEN3_7379_GATCAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WEN3_7379_GATCAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43167728
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA33250.029.30179241
CTCGTAT33950.028.5680742
TCGTATG34950.027.5618943
CGTATGC38350.025.34971844
ACGATCA43250.022.43217532
CGATCAG45100.021.6095733
ATCTCGT47650.020.49283840
GTCACGA49100.019.71469729
CACGATC51150.019.1396231
GATCTCG51450.019.0643539
TCACGAT51200.019.03499230
GATCGGA55900.017.7918871
ATCGGAA56800.017.7739432
CGTCTGA57550.016.9671816
ACACGTC65000.015.5300513
AGTCACG63900.015.49278828
ACGTCTG67100.014.88013415
TCGGAAG80750.012.5295733
CGGAAGA84850.012.08000854
CACGTCT86450.012.00744214
Produced by FastQC (version 0.11.2)