FastQCFastQC Report
Tue 1 Mar 2016
input_WEN2_6145_CTTGTA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WEN2_6145_CTTGTA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43538313
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3205130.7361631122455295TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT351350.042.05050742
TCTCGTA351300.041.9388141
TCGTATG353700.041.82657243
CGTATGC354350.041.7312244
AATCTCG357150.041.4126939
GATCGGA368900.041.0140881
ATCTCGT363300.040.64428740
ATCGGAA373450.040.572082
CGTCTGA370050.040.49284716
ACACGTC378700.039.86998713
ACGTCTG377000.039.81054715
TCGGAAG394050.038.4286043
CGGAAGA400450.037.792474
CACGTCT400900.037.6621714
GTAATCT393800.037.57908637
TTGTAAT406500.036.58902735
CACACGT415900.036.48885312
TGTAATC416350.035.67585436
GCACACG431900.035.32557311
TAATCTC424500.034.91916338
Produced by FastQC (version 0.11.2)