FastQCFastQC Report
Tue 1 Mar 2016
input_WEN1_6139_ACTTGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameinput_WEN1_6139_ACTTGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39465967
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3587580.9090313180467615TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT393250.042.3575742
TCGTATG394400.042.26741443
TCTCGTA395250.042.1760941
CGTATGC395950.042.09084344
AATCTCG404000.041.49827639
ATCTCGT406800.041.05029740
GATCGGA443850.038.4256821
ATCGGAA448000.038.1530762
TTGAATC442350.038.0479136
CGTCTGA450200.037.83600616
ACACGTC455450.037.40331613
ACGTCTG456250.037.33768515
TCGGAAG465500.036.7281043
GAATCTC461300.036.5101938
CTTGAAT465450.036.25874735
CGGAAGA472600.036.2042474
TGAATCT465350.036.1532137
CACGTCT477300.035.76011714
CACACGT488050.035.01874512
GCACACG506150.033.83602511
Produced by FastQC (version 0.11.2)