FastQCFastQC Report
Tue 1 Mar 2016
K9me3_WNN3_7732_NCTTGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK9me3_WNN3_7732_NCTTGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22115620
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29800.034.84521542
TCGTATG30150.034.6596143
TCTCGTA31050.033.58170741
CGTATGC32600.032.12470644
AATCTCG38100.027.59728439
ATCTCGT37800.027.58497240
CGTCTGA50250.021.33271216
GATCGGA53650.019.884911
ATCGGAA53600.019.8212032
ACGTCTG54850.019.4633815
TTGAATC56750.018.87910836
ACACGTC57000.018.69061913
TCGGAAG62800.017.092593
TGAATCT63350.017.0855937
GAATCTC63850.016.6067638
CGGAAGA65650.016.3173924
CTTGAAT68050.015.9703835
CACGTCT70000.015.25094914
CACACGT72050.014.84035312
GTCACAC84250.012.794373529
Produced by FastQC (version 0.11.2)