FastQCFastQC Report
Tue 1 Mar 2016
K9me3_WNN3_7732_ACTTGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK9me3_WNN3_7732_ACTTGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25544259
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG32600.035.35886443
CTCGTAT33000.034.9302742
TCTCGTA33600.034.1746441
CGTATGC34300.033.60756744
ATCTCGT40250.028.637740
AATCTCG40950.028.25416639
CGTCTGA57850.020.31911816
ACGTCTG61000.019.19768115
GATCGGA61750.018.9501821
ATCGGAA62200.018.7423572
TTGAATC63450.018.62037836
ACACGTC66950.017.45865813
GAATCTC71600.016.4389138
TCGGAAG72550.016.3111113
TGAATCT73700.015.911139537
CTTGAAT78200.015.36143235
CGGAAGA76700.015.2566514
CACGTCT80650.014.73860414
CACACGT83200.014.22674812
TTCGGTA7150.012.30531325
Produced by FastQC (version 0.11.2)