FastQCFastQC Report
Tue 1 Mar 2016
K9me3_WNN1_7728_TTAGGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK9me3_WNN1_7728_TTAGGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42900138
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT33450.031.76648542
TCTCGTA34050.030.94683841
TCGTATG34150.030.92207743
CGTATGC36850.029.25635744
TATTCGT18100.025.521651
TAAGCCG32600.022.81816716
CCGATGA32950.022.6313420
ATCTCGT49100.021.72974240
CGATGAC34000.021.60894421
AGCCGAT34400.021.36559518
TTCGGTA24900.021.11378325
TCGGTAT25550.020.4905526
CGGTATT26650.019.64478327
AATTCGG27650.019.33203723
CATCTCG57600.018.52208139
GATCGGA59000.018.2316021
ATTCGGT29200.017.85389524
GATGACG41300.017.84270922
ATCGGAA61650.017.3765642
GAATTCG31950.017.2121522
Produced by FastQC (version 0.11.2)