FastQCFastQC Report
Tue 1 Mar 2016
K9me3_WEN3_7737_ATGTCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK9me3_WEN3_7737_ATGTCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39395290
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT43100.034.7083744
TCTCGTA44900.033.3168243
AATCTCG53500.028.08086841
ATCTCGT54500.027.56726642
CGTCTGA88050.017.4025916
GATCGGA90600.016.5332241
ATCGGAA90950.016.517972
ACGTCTG95200.016.07244315
ACACGTC98050.015.69508213
GAATCTC107250.014.33583440
TCGGAAG111750.013.5812643
CACGTCT114200.013.47550714
CGGAAGA119450.012.761034
TTCGGTA12250.012.74855225
CACACGT127200.012.03783612
TCGGTAT13400.011.65446126
TATTCGT11800.011.3706811
ACATGTC138750.011.30497532
GTCACAT140800.011.18674129
AGAATCT140700.011.08402239
Produced by FastQC (version 0.11.2)