FastQCFastQC Report
Tue 1 Mar 2016
K9me3_WEN1_7727_ATCACG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK9me3_WEN1_7727_ATCACG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39046752
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA439420.11253688911180115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT35600.034.4785461
CCGATGA62400.033.70054620
TAAGCCG62250.033.6583816
AGCCGAT63850.033.05144518
CGATGAC66150.031.98960521
CTCGTAT28750.031.44982142
GATGACG70950.030.19744322
TCGTATG29800.030.19404443
TCTCGTA30400.029.88627641
CGTATGC30400.029.81906744
TCATAAC74100.028.44041429
CGGTATT51000.028.29419327
TCGGTAT51300.028.21448526
TTCGGTA51300.028.08584825
ACGTCAT75450.027.87162426
AAGCCGA77600.027.3407817
ATGACGT78550.027.3317423
AATTCGG54850.026.30818423
ATAACAT81750.025.83571631
TATAAGC82150.025.8264714
Produced by FastQC (version 0.11.2)