FastQCFastQC Report
Tue 1 Mar 2016
K79me2_WNN3_7521_GCCAAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK79me2_WNN3_7521_GCCAAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41647042
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1335550.32068303914597346TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT151550.040.18947642
TCTCGTA152100.040.06020441
TCGTATG153050.039.8357143
CGTATGC155700.039.1391644
CGCCAAT158300.038.85685733
TATCTCG160350.038.2050939
GATCGGA168500.037.139911
ATCGGAA168550.037.1066062
ATCTCGT164500.037.06735640
ACGCCAA167100.036.83669332
GTCACGC170050.036.17386629
CCAATAT172800.035.7708335
CGTCTGA175050.035.37283316
CAATATC175400.035.12767436
GCCAATA178900.034.50086234
ACACGTC180800.034.30894513
ACGTCTG181750.034.1175115
AGTCACG181600.033.91007628
TCACGCC188650.032.67569730
TCGGAAG192200.032.586483