FastQCFastQC Report
Tue 1 Mar 2016
K4me3_WNN3_7369_TAGCTT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me3_WNN3_7369_TAGCTT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44319101
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2608490.5885701517275812TruSeq Adapter, Index 10 (100% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA520980.1175520234492121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG290950.041.089243
CTCGTAT292200.040.9284842
CGTATGC295000.040.5553944
TATCTCG297550.040.2514939
TCTCGTA299450.039.93021441
ATCTCGT306850.038.9814240
TAGCTTA319350.037.64471434
GCTTATC323650.037.12074736
AGCTTAT326550.036.82734335
ATCGGAA343100.036.009982
ACACGTC343250.035.86469313
GTCACTA337000.035.75798429
TATTCGT42200.035.7102471
TTATCTC339150.035.48284538
CGTCTGA346600.035.44426716
CTTATCT340950.035.300737
GATCGGA353100.035.047411
CGTCATA66500.034.8963727
ACTAGCT348000.034.72886332
ACGTCTG357100.034.45396415
Produced by FastQC (version 0.11.2)