FastQCFastQC Report
Tue 1 Mar 2016
K4me3_WEN3_7371_CAGATC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me3_WEN3_7371_CAGATC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43636038
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1566110.3589028866461249TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG174700.040.57431443
CTCGTAT177400.040.01876442
CGTATGC181200.039.22252344
TCTCGTA183450.038.7349541
ATCTCGT189950.037.37472540
ATCGGAA216250.033.9634862
CGTCTGA221150.032.89764816
GATCATC219400.032.41966636
AGATCAT220700.032.33584635
ACACGTC225000.032.2272813
GATCGGA231250.031.8027651
ACGTCTG230350.031.57426315
CATCTCG230400.030.97533439
CGTCATA33100.030.83305527
ATCATCT236000.030.29623837
CACGTCT241450.030.11361314
CACACGT252450.028.89730612
TATTCGT21150.028.3517021
ACCAGAT256750.028.12319832
TCGGAAG265600.027.7773273
Produced by FastQC (version 0.11.2)