FastQCFastQC Report
Tue 1 Mar 2016
K4me1_WNN3_8269_CTTGTA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me1_WNN3_8269_CTTGTA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37467620
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4830801.289326623895513TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT528350.042.07394842
TCTCGTA531800.041.80771341
CGTATGC537850.041.4128844
TCGTATG539750.041.2956543
ATCTCGT541200.041.09964840
GTAATCT541800.040.83395837
AATCTCG543300.040.79609739
TTGTAAT552100.040.1797535
TGTAATC555650.039.86636736
CTTGTAA565200.039.2926734
CGTCTGA594900.039.25560816
TAATCTC564750.039.19585438
ACACGTC601250.038.91304813
ACGTCTG601750.038.88551315
CCTTGTA581100.038.3106133
ATCGGAA626900.037.6656762
GATCGGA623600.037.6426661
CACGTCT623200.037.5954214
CACACGT624000.037.5114212
ACCTTGT601600.037.0037632
Produced by FastQC (version 0.11.2)