FastQCFastQC Report
Tue 1 Mar 2016
K4me1_WNN1_8262_TTAGGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me1_WNN1_8262_TTAGGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35953848
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC489860.13624689073614596TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT61600.036.703142
TCTCGTA63200.035.9132441
TCGTATG69550.032.5711943
ATCTCGT71450.031.79629540
CGTATGC72850.031.2770844
ATCGGAA78650.030.0977942
GATCGGA79900.029.6409051
CGTCTGA84050.027.87290616
ACACGTC85250.027.68579313
TATTCGT18550.027.1717431
CATCTCG86500.026.3890839
ACGTCTG90200.026.02118315
TCGGAAG99000.024.0413823
CACGTCT100700.023.48228314
CCGATGA37400.023.29082720
TAAGCCG37500.023.17054216
CGTCATA38900.022.56139227
CGGAAGA106250.022.4203364
AGCCGAT39000.022.16637618
ACGTCAT40200.021.7223626
Produced by FastQC (version 0.11.2)