FastQCFastQC Report
Tue 1 Mar 2016
K4me1_WEN3_8261_CGTACG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me1_WEN3_8261_CGTACG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32819452
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1648800.5023849880247848TruSeq Adapter, Index 22 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA721650.21988484146536025No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT457450.13938380202082595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC184750.041.34053438
TACGTAA186750.041.0283136
CGTACGT187900.040.89937634
CTCGTAT188050.040.82494444
GTACGTA188400.040.7243935
ACGTAAT188100.040.71448537
TCTCGTA188950.040.65378643
CCGTACG191350.040.3552933
TATTCGT53750.039.8185581
ACCGTAC197200.039.2115732
AATCTCG196050.039.1246441
ATCTCGT199100.038.54805442
CACCGTA202600.038.19883731
GTAATCT200550.038.1430439
ATCGGAA222500.037.22022
CGTCATA90700.036.68613427
ACACGTC223750.036.6644513
CGTCTGA223400.036.66310516
GATCGGA226800.036.3191381
ACGTCTG232850.035.27960215
Produced by FastQC (version 0.11.2)