FastQCFastQC Report
Tue 1 Mar 2016
K4me1_WEN1_8297_GATCAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4me1_WEN1_8297_GATCAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43334755
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3490750.8055312646858163TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA399800.041.775591
CTCGTAT388450.041.75444442
TCTCGTA388200.041.7347641
ATCGGAA401900.041.714792
ACGATCA387350.041.5061832
CGATCAG390600.041.1669833
CACGATC392300.040.99296631
TCACGAT393050.040.9651130
GTCACGA394250.040.9073729
ATCTCGT396900.040.81321740
TCGTATG398600.040.77975543
CGTATGC399850.040.65777644
ACACGTC411300.040.502213
GATCTCG396700.040.4745139
CGTCTGA412000.040.4398916
ACGTCTG421000.039.5857915
TCGGAAG425750.039.379043
CACGTCT427450.039.02443714
AGTCACG413950.038.77459728
CGGAAGA434450.038.575274
Produced by FastQC (version 0.11.2)