FastQCFastQC Report
Tue 1 Mar 2016
K4m3_WNN2_6550_ACTGAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4m3_WNN2_6550_ACTGAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39084091
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT761410.19481328093315511TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT90500.038.69694544
TATCTCG93850.037.43380741
TCTCGTA97000.036.19493543
ATATATC103550.033.96991738
ATCTCGT103900.033.6856742
TATATCT113450.031.06376639
GATATAT115950.030.52676837
ACTGATA118300.029.92005734
ATATCTC119000.029.70742640
TGATATA120500.029.33762436
ATCGGAA127600.028.3836822
ACACGTC129350.027.94205713
CGTCTGA129350.027.92476316
CTGATAT132000.026.9481835
GATCGGA137500.026.4685611
ACGTCTG138600.026.14045515
TATTCGT19150.025.0638561
CACGTCT149350.024.34727714
CGTCATA27850.023.9331127
CACACGT155050.023.55177112
Produced by FastQC (version 0.11.2)