FastQCFastQC Report
Tue 1 Mar 2016
K4m3_WNN1_6545_GTTTCG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4m3_WNN1_6545_GTTTCG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37946435
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1179180.31074855912024407TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT129300.040.49088344
TCTCGTA136550.038.37327243
AATCTCG142550.036.85313841
ATCTCGT143200.036.69899742
CGTTTCG146450.036.0657333
TCGGAAT147200.035.80890337
ACGTTTC160000.033.17574732
TTCGGAA160650.032.9753136
TTTCGGA161550.032.90054735
CGGAATC166150.031.68504538
GTTTCGG170450.031.14241234
ATCGGAA177100.030.5150012
CGTCTGA176200.030.49983616
ACACGTC176100.030.41790413
ACGTCTG180150.029.87993415
GAATCTC177400.029.58858940
AGTCACG182400.029.14946628
GTCACGT183850.028.93157229
GATCGGA190150.028.5237831
CACGTCT194950.027.69114914
Produced by FastQC (version 0.11.2)