FastQCFastQC Report
Tue 1 Mar 2016
K4m3_WEN2_6543_AGTCAA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4m3_WEN2_6543_AGTCAA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36043505
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT904410.25092176801340493TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT104400.039.65476244
TCTCGTA111450.037.1858543
ATCTCGT116200.035.62845242
AATCTCG118600.035.05765541
AACAATC123250.033.69971538
ACAATCT126900.032.81709739
CAACAAT131700.031.7379637
AGTCAAC135450.030.98792334
GTCAACA138150.030.36776235
ATCGGAA144650.030.0720442
ACACGTC145800.029.7533513
TCAACAA142000.029.6063836
TATTCGT18000.029.1051981
CGTCTGA150250.028.9301816
CAATCTC146900.028.43864640
CAGTCAA149450.028.1439533
ACGTCTG155400.028.0705115
GATCGGA157250.027.7726381
CGTCATA30550.027.21803727
CACACGT168250.026.01863312
Produced by FastQC (version 0.11.2)