FastQCFastQC Report
Tue 1 Mar 2016
K4m3_WEN1_6558_ACTGAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK4m3_WEN1_6558_ACTGAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37306605
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT5815081.5587266651575507TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG402740.10795407408420038TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA670000.040.364771
CTCGTAT662650.040.17436644
TATCTCG668050.039.82260541
TCTCGTA668200.039.79667343
ATCTCGT675700.039.36502542
ATATATC681400.039.10098338
GATATAT690000.038.6487137
TATATCT689550.038.60693439
ATATCTC694550.038.3606840
ACACGTC701350.038.35811213
ATCGGAA705350.038.3386882
CGTCTGA701350.038.31420516
ACTGATA700150.038.1380334
TGATATA702450.038.0259436
ACGTCTG709600.037.87185315
CACGTCT721050.037.3040214
CTGATAT720250.037.11076735
CACACGT736750.036.6496512
TCGGAAG739050.036.593463
CGGAAGA747150.036.224724
Produced by FastQC (version 0.11.2)