FastQCFastQC Report
Tue 1 Mar 2016
K36m3_WNN2_6585_CAGATC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK36m3_WNN2_6585_CAGATC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46700449
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC622090.13320856936514677TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT79200.035.36137842
TCGTATG79850.035.10107843
TCTCGTA79950.034.8645541
CGTATGC84100.033.22242444
ATCTCGT97050.028.85747540
CATCTCG113700.024.96074339
TATTCGT21450.022.4663281
CGTCATA38750.022.42506227
GATCATC138550.020.62655436
TAAGCCG42850.020.17688816
CGGTATT28900.020.02009827
TCGGTAT29050.019.61380826
TTCGGTA29100.019.50450725
CGTCTGA150350.019.31447816
AGCCGAT45000.019.2124518
AGATCAT155450.018.68126735
ACACGTC160300.018.04739213
ACGTCTG163300.017.9310515
ACGTCAT49100.017.92201226
AATTCGG31900.017.86134323
Produced by FastQC (version 0.11.2)