FastQCFastQC Report
Tue 1 Mar 2016
K36m3_WNN1_6579_CGATGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK36m3_WNN1_6579_CGATGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50913924
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66850.032.78045742
TCGTATG67450.032.5543543
TCTCGTA69250.031.64429341
TATCTCG71000.031.05040439
CGTATGC72400.030.20681444
CGATGTA77250.028.48099734
ATCTCGT85150.025.8125140
CACCGAT91700.024.28035531
CCGATGT92950.023.76500933
ACCGATG94900.023.46169932
TATTCGT20250.022.9274791
TCACCGA114450.019.6654230
GATGTAT113350.019.56557835
CGTCATA35450.019.54821227
CGGTATT28600.019.23032827
TCGGTAT28600.018.99904326
TTCGGTA29300.018.4699325
GTATCTC126450.017.85200138
GTCACCG129300.017.61101529
TAAGCCG42400.016.49924916
Produced by FastQC (version 0.11.2)