FastQCFastQC Report
Tue 1 Mar 2016
K36m3_WEN2_6576_TTAGGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK36m3_WEN2_6576_TTAGGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51351291
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA596850.11622882080997729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT45600.036.636311
TCGTATG66700.034.73134643
CTCGTAT66850.034.71923442
TCTCGTA71400.032.4759241
CGTATGC72150.032.26020444
TAAGCCG86350.030.44539816
CCGATGA86750.030.43128420
AGCCGAT87550.030.07782718
TCGGTAT66900.029.4311426
TTCGGTA67250.029.40870325
CGGTATT67650.029.2024427
CGTCATA90350.029.1701927
AATTCGG68450.028.86123
CGATGAC93300.028.38921221
TCATAAC94150.028.10968229
ATTCGGT70050.028.10757624
GAATTCG72300.027.35456822
ACGTCAT96350.027.3536526
ATCTCGT85800.027.28182440
GGATTTA99700.026.721589
Produced by FastQC (version 0.11.2)