FastQCFastQC Report
Tue 1 Mar 2016
K36m3_WEN1_6571_CGATGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK36m3_WEN1_6571_CGATGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48234909
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA904410.18750113118281203No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC615840.12767516571867069TruSeq Adapter, Index 2 (100% over 50bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT539050.11175516056223928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT61200.037.4338341
CTCGTAT79150.036.93945742
TCGTATG80450.036.5066243
CGTCATA116400.035.36104227
TCTCGTA83950.034.90598741
TATCTCG86000.034.3297439
CGTATGC86850.033.9430944
TAAGCCG121350.033.91989516
CGATGTA89300.033.28286734
AGCCGAT124150.033.1371718
CCGATGA127250.032.3981820
CGATGAC128500.032.13404521
ATGACGT129100.032.10398523
ACGTCAT131600.031.4773926
TCATAAC136550.030.25595329
GTCATAA137550.030.06788428
GATGACG141400.029.34246322
TATAAGC142050.029.20928414
ATCTCGT101650.029.17419840
CACCGAT102500.028.95330831
Produced by FastQC (version 0.11.2)